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  • GeneName: CD74; II; DHLAG; HLADG; Ia-GAMMA; Ii.
  • SynName: Monoclonal anti-human CD74/FITC.
  • CD74 molecule, major histocompatibility complex, class II invariant chain.
  • Clonality: Monoclonal; *Isotype: IgG2a; *Clone: M-B741; *Source: Mouse; *Reactivity: Human.
  • Tested: Flow Cytometry (FC/FACS); *AppNotes: 1:50 (1.6mul conc stock per 5 x 105 cell test).
  • Form: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA..
  • Conc: Volume: 0.2 ml.
  • Conjugation: FITC.
  • Immunogen: RPMI 1788 cell line.
  • FREE-8GB-USBDrive for this item. Item is for research use. NOT for diagnostic/therapeutic procedure..
  • Imported from USA.
SynName: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); *Performance: Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD74/FITC at a 1:50 dilution. Cells were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. This shift was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD74 antibody.; *Production: Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at approximately 0.5 mg/ml with a Fluorescein:Ab molar ratio of 7.7.; *Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).; *Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light Product should retain activity for at least 12 months after shipping date when stored as recommended.; *Accession#: AAH24272.1; *Description: Information: Human CD74 is expressed intracellularly and on the cell surface of MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans cells and dendritic cells. CD74 has several important functions relating to antigen presentation by MHC Class II molecules. Antibody M-B741 recognizes the CD74 molecule of about 33 kd.; *NCBI Summary: The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with ...

anti-human CD74-FITC (Antibody)


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