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Description

  • Kit contains 2 Maxi GD columns, (2) filter column, 10mL GP1 buffer, 10mL GPX1 buffer, 3mL GP2 buffer, 8mL GP3 buffer (16mL add isopropanol).
  • It also contains 10mL W1 buffer, 5mL wash buffer (20mL add ethanol), 6mL elution buffer and 100 microliter RNASE A (10mg/mL).
  • For research use only, extract genomic from and fungal tissues.
  • Sample size: 1g of fresh or 250mg of dry tissue.
  • Spin column format.
The Genomic maxi kit () provides an efficient method for purifying total (genomic , mitochondrial and chloroplast) from tissue and cells. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, the genomic in the lysate binds to the glass fiber matrix of the spin column (1). Contaminants are removed using a wash buffer (containing ethanol) and the purified genomic is eluted by a low salt elution buffer or TE. The procedure does not require phenol extraction or alcohol precipitation, and can be completed in less than 1 hour. The purified genomic is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP. The quality of the Genomic maxi kit () is tested on a lot-to-lot basis by isolating genomic from 250 mg young leaf samples. More than 50 microgram of genomic is quantified with a spectrophotometer and checked by electrophoresis. Caution: Some components are irritants. During operation, always wear a lab coat, disposable gloves, and protective goggles. Operation: centrifuge/vacuum manifold. Binding capacity: 500 microgram. Expectant yield: up to 140 microgram of . Operation time: 60 minutes.

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IBI Scientific IB47242 Maxi Genomic Sample Kit for 2 Preparations

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