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Description

  • Kit contains (4) SD columns, (4) 2mL collection tubes, 3mL SD buffer, 2mL W1 buffer, 1mL wash buffer (4mL add Ethanol), 1mL elutiok buffer.
  • For research use only.
  • Small fragment extraction kits were designed to recover or concentrate fragments (50-200bp) from an agarose gel, PCR, or other enzymatic reactions.
  • Chaotropic salt is used to dissolve agarose gel and denature enzymes.
  • Sample: up to 300 mg of agarose gel and up to 100 microliter of PCR products.
The small fragments extraction kit was designed to recover or concentrate fragments (50-200 bp) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column (1). Contaminants are removed with a Wash Buffer (containing ethanol) and the purified fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90 percent for Gel extraction and up to 95 percent for PCR clean up. The entire procedure can be completed in 20 minutes and the eluted is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, Labeling and Ligation. The quality of the Small Fragments Extraction Kit is tested on a lot-to-lot basis by isolating fragments of various sizes from either aqueous solutions or agarose gel. The purified is checked by electrophoresis. Caution: SD Buffer contains guanidine thiocyanate which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, and protective goggles. Recovery: up to 95 percent. Format: spin column. Operation time: 20 minutes. Elution volume: 20-50 microliter. Applications: PCR, fluorescent or radioactive sequencing, restrictive digestion, labeling, ligation. Operation: centrifuge/vacuum manifold.

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IBI Scientific IB47060 Gel/PCR Small Fragment Extraction Sample Kit for 4 Preparations

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