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Description

  • The quality of the Soil Extraction Kit is tested on a lot-to-lot basis by isolating genomic from 250 mg soil samples.
  • The purified genomic is ready for use in pcr, restriction enzyme digestion, and sequencing reactions.
  • Pack of 4.
The Soil Extraction Kit was designed for raid isolation of genomic from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is genized and disrupted using SL1 (lysis) Buffer combined with ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic are then precipitated with a unique inhibitor removal buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic is bound by the GD Column. The column is then washed and the is eluted with Elution Buffer. phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes.

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IBI Scientific IB47800 Soil Extraction Prep Kit (Pack of 4)

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